Product Details
Synonyms
BTN3A1, CD277, BTF5
Source
Human BTN3A1 Protein, His Tag (BT1-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Gly 254 (Accession # O00481-1).
Predicted N-terminus: Gln 30
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.5 kDa. The protein migrates as 32-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human BTN3A1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Human BTN3A1 Protein, His Tag (Cat. No. BT1-H52H4) is more than 85% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human BTN3A1 Protein, His Tag (Cat. No. BT1-H52H4) at 1 μg/mL (100 μL/well) can bind Human BTN3A1/2/3 Antibody with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Butyrophilin subfamily 3 member A1
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
1 Details
- Highest Development Stage:
Phase 2 Clinical
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