Cynomolgus APRIL / TNFSF13 Protein, His,Flag Tag, active trimer, low endotoxin (MALS verified)

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제품 번호/규격
가격
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APL-C52D2-100ug
$415.00
APL-C52D2-1mg
$3140.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    APRIL, TNFSF13, TALL-2, TRDL-1, CD256, TALL2, ZTNF2

  • Source

    Cynomolgus APRIL, His,Flag Tag (APL-C52D2) is expressed from human 293 cells (HEK293). It contains AA Gln 111 - Leu 250 (Accession # XP_005582818.1) trimer Design.

    Predicted N-terminus: His

  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

    The protein has a calculated MW of 50.4 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

고객님도 확인한 상품

데이터 표시

  • SDS-PAGE

    APRIL SDS-PAGE

    Cynomolgus APRIL, His,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    APRIL SEC-MALS

    The purity of Cynomolgus APRIL, His,Flag Tag (Cat. No. APL-C52D2) is more than 95% and the molecular weight of this protein is around 57-69 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     APRIL ELISA

    Immobilized Cynomolgus APRIL, His,Flag Tag (Cat. No. APL-C52D2) at 1 μg/mL (100 μL/well) can bind Human TACI (R119A, R122A), Fc Tag (Cat. No. TAI-H5253) with a linear range of 2-13 ng/mL (QC tested).

    Protocol
  •  APRIL ELISA

    Immobilized Cynomolgus APRIL, His,Flag Tag (Cat. No. APL-C52D2) at 1 μg/mL (100 μL/well) can bind Human BCMA, Fc Tag (HPLC verified) (Cat. No. BC7-H5254) with a linear range of 0.1-1 ng/mL (Routinely tested).

    Protocol

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배경 소개

APRIL(a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFSF13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Tumor necrosis factor ligand superfamily member 13

  • 카테고리:

  • 상장 의약품 수:

    2 상세

  • 임상 의약품 수:

    30 상세

  • 최고 개발 단계:

    Approved

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