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Product Details
Synonyms
ALPG, GCAP, ALP-1, ALPPL2, PLAP-like, ALPPL, Alkaline phosphatase, germ cell type
Source
Human ALPG, His Tag (ALG-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Asp 503 (Accession # P10696-1).
Predicted N-terminus: Ile 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 54.5 kDa. The protein migrates as 60-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human ALPG, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ALPG, His Tag (Cat. No. ALG-H52H9) is more than 90% and the molecular weight of this protein is around 97-119 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human ALPG, His Tag (Cat. No. ALG-H52H9) at 1 μg/mL (100 μL/well) can bind Anti - ALPG / ALPPL2 with a linear range of 0.002-0.125 μg/mL (QC tested).
Protocol
Bioactivity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >5000 pmol/min/μg (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Alkaline phosphatase
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Approved
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