Human / Cynomolgus Activin RIIA / ACVR2A Protein, His Tag, low endotoxin (MALS & SPR verified)

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제품 번호/규격
가격
수량
ACA-H5243-200ug
$345.00
ACA-H5243-1mg
$1275.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    Activin receptor type IIA, ACTRIIA

  • Source

    Human / Cynomolgus Activin RIIA Protein, His Tag (ACA-H5243) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 135 (Accession # P27037-1). In the region Ala 20 - Pro 135, the amino acid sequences of Human and Cynomolgus Activin RIIA are identical.

    Request for sequence
  • Molecular Characterization

    Activin RIIA Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 15.4 kDa. The protein migrates as 29-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    Activin RIIA SDS-PAGE

    Human / Cynomolgus Activin RIIA Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Activin RIIA SEC-MALS

    The purity of Human / Cynomolgus Activin RIIA Protein, His Tag (Cat. No. ACA-H5243) is more than 95% and the molecular weight of this protein is around 18-28 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Activin RIIA ELISA

    Immobilized Human / Cynomolgus Activin RIIA Protein, His Tag (Cat. No. ACA-H5243) at 1 μg/mL (100 μL/well) can bind Bimagrumab with a linear range of 0.05-1 ng/mL (Routinely tested).

    Protocol
  • Bioactivity-SPR

     Activin RIIA SPR

    Human / Cynomolgus Activin RIIA Protein, His Tag (Cat. No. ACA-H5243) immobilized on CM5 Chip can bind Human Activin A Protein, premium grade (Cat. No. ACA-H5314) with an affinity constant of 85.9 pM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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배경 소개

Activin receptor type-2A (ACVR2A) is also known as activin receptor type IIA, ACTR-IIA, ACTRIIA, and ACVR2, and is a single-pass type I membrane protein. ACVR2A belongs to the protein kinase superfamily, the TKL Ser/Thr protein kinase family, and the TGFB receptor subfamily. Upon ligand binding, ACVR2A forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors, which then phosphorylate and activate SMAD transcriptional regulators. ACVR2A also serves as a receptor for activin A, activin B, and inhibin A (the latter in complex with betaglycan). Two type II activin receptors (ACVR2A and ACVR2B) have been identified, which bind different activins with distinct affinities.

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Activin receptor type-2A

  • 카테고리:

  • 상장 의약품 수:

    0 상세

  • 임상 의약품 수:

    4 상세

  • 최고 개발 단계:

    Phase 2 Clinical

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