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Product Details
Product Overview
Human Tumor Necrosis Factor Alpha(TNF-α) ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TNF-alpha that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteTNF-αFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity2.321 pg/mLRange19.5 pg/mL-1250 pg/mLAssay Time2 hr 50 minSample volume100 μLSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human TNF-alpha in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCRS002-C01Pre-coated Anti-TNF-α Antibody Microplate1 plateCRS002-C02Human TNF-α Standard20 μgCRS002-C03Biotin-Anti-TNF-α Antibody20 μgCRS002-C04Streptavidin-HRP50 μLCRS002-C0510xWashing Buffer50 mLCRS002-C062xDilution Buffer50 mLCRS002-C07Substrate Solution12 mLCRS002-C08Stop Solution7 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of TNF-α is less than 2.321 pg/mL.
Sample Values
Serum Sample: Samples from apparently healthy volunteers were evaluated for the presence of human TNF-α in this assay. No medical histories were available for the donors used in this study.
Cell Culture Supernatant: Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated using CD28/OKT3 antibody then detected TNF-α secretion amount.
Dilution Linearity
High concentrations (1200pg/mL) of human TNF-α were added to different diluted substrates for gradient dilution to evaluate the linearity of the assay.
Intra-Assay Statistics
Twenty replicates of each of three samples containing different TNF-α concentrations were tested in one assay. Acceptable criteria: CV＜15%.
Inter-Assay Statistics
Three samples containing three different concentrations of TNF-α were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
TNF-α was spiked into three human serum samples, and then analyzed. The average recovery of TNF-α for serum samples is 101.0%, range is 89.6% - 111.4%.
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Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor α
- Category：
- Approved Drugs：
68 Details
- Drugs in Clinical Trials：
74 Details
- Highest Development Stage：
Approved
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