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Product Overview
Human TARC/CCL17 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TARC/CCL17 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteCCL17Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity3.0 pg/mLAssay Time1.75 hrSample volume50 μLRange31.25pg/mL-2000 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human TARC/CCL17 in serum, plasma and cell culture supernatants. It is for research use only.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA029-C01Pre-coated Anti-CCL17 Antibody Microplate1 plateCEA029-C02Human CCL17 Standard500 μLCEA029-C03Biotin-Anti-CCL17 Antibody Con. Solution100 μLCEA029-C04Biotin-Antibody Dilution Buffer8 mLCEA029-C05Streptavidin-HRP Con. Solution0.5 mLCEA029-C06Streptavidin-HRP Dilution Buffer15 mLCEA029-C0720×Washing Buffer50 mLCEA029-C08Sample Dilution Buffer15 mL×2CEA029-C09Substrate Solution12 mLCEA029-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of CCL17 is less than 3 pg/mL.
Sample Values
Serum Sample: TARC/CCL17 concentrations were measured in thirty-two human serum samples, which were collected from healthy human subjects. The measurements of thirty-two samples are in the range of 1000-4000pg/mL and shown in the figure below.
Cell Culture Supernatant: Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated using 10 μg/mL PHA, then detected CCL17 secretion amount on Day1, Day 3 and Day 5.
Matrix Effect
Three types of materials were tested to observe if there were matrix effect (interference). If the concentration of hemoglobin (simulated hemolysis) is less than 3500 mg/dL(2%), the concentration of triglyceride (simulated lipid blood) is less than 3 mg/mL, testing results will not be affected.
Dilution Linearity
High concentrations of human TARC/CCL17 serum samples were diluted with 1:2, 1:4, 1:8 and 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of TARC/CCL17 was 97.89%.
Intra-Assay Statistics
Ten replicates of each of three samples containing different TARC/CCL17 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three lots of samples containing different concentrations of TARC/CCL17 were tested in the independent assays. Acceptable criteria: CV<15%.
Recovery
TARC/CCL17 was spiked into three human serum samples, and then analyzed. The average recovery of TARC/CCL17 for serum samples is 93.93%.
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