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Product Overview
Human IL-6 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-6 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-6Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity2.0 pg/mLAssay Time1.75 hrSample volume50 μLRange6.25 pg/mL-400 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code89/548Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human IL-6 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCRB001-C01Pre-coated Anti-IL-6 Antibody Microplate1 plateCRB001-C02Human IL-6 Standard20 μg×2CRB001-C03Biotin-Anti-IL-6 Antibody Con. Solution100 μLCRB001-C04Biotin-Antibody Dilution Buffer8 mLCRB001-C05Streptavidin-HRP Con. Solution500 μLCRB001-C06Streptavidin-HRP Dilution Buffer15 mLCRB001-C0720×Washing Buffer50 mLCRB001-C081×Dilution Buffer15 mL×2CRB001-C09Substrate Solution12 mLCRB001-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-6 is less than 2.0 pg/mL.
Sample Values
Serum Sample: two hundred and forty samples from healthy volunteers were tested for the presence of human IL-6 concentration in the assay. One hundred and eighty-seven samples were detectable. No medical histories were available for the donors used in this study.
Cell Culture Supernatant: Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated with target cells at E:T ratios of 1:10 and 1:5, and then detect the secretion of IL-6 under stimulation with different concentrations of Blinatumomab.
Matrix Effect
Five types of materials were tested to observe if there were matrix effect (interference). If the concentration of bilirubin (simulated jaundice) is less than 20 mg/dL, the concentration of triglyceride (simulated lipid blood) is less than 3.0 mg/mL, the concentration of hemoglobin (simulated hemolysis) is less than 2% v/v, testing results will not be affected.
Dilution Linearity
High concentrations of human IL-6 serum samples were diluted with 1:2, 1:4, 1:8, 1:16, 1:32 and 1:64 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of IL-6 was 98.43%.
Intra-Assay Statistics
Ten replicates of each of three samples containing different IL-6 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of IL-6 were tested in the independent assays. Acceptable criteria: CV<15%.
Recovery
IL-6 was spiked into three human serum samples, and then analyzed. The average recovery of IL-6 for serum samples is 94.64%.
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프론티어 진행
의약품 개발 진행- 영어 이름：
Interleukin-6
- 카테고리：
- 상장 의약품 수：
5 상세
- 임상 의약품 수：
22 상세
- 최고 개발 단계：
Approved
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