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Product Overview
Human GM-CSF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human GM-CSF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteGM-CSFFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity10 pg/mLAssay Time1.75 hrSample volume50 μLRange12.5 pg/mL-1600 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code88/646Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human GM-CSF in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCRB007-C01Pre-coated Anti-GM-CSF Antibody Microplate1 plateCRB007-C02Human GM-CSF Standard32 μg×2CRB007-C03Biotin-Anti-GM-CSF Antibody Con. Solution100 μLCRB007-C04Biotin-Antibody Dilution Buffer8 mLCRB007-C05Streptavidin-HRP Con. Solution500 μLCRB007-C06Streptavidin-HRP Dilution Buffer15 mLCRB007-C0720×Washing Buffer50 mLCRB007-C081×Dilution Buffer15 mL×2CRB007-C09Substrate Solution12 mLCRB007-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of GM-CSF is less than 10.0 pg/mL.
Intra-Assay Statistics
Ten replicates of each of three samples containing different GM-CSF concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of GM-CSF were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant GM-CSF was spiked into five human serum samples, and then analyzed. The average recovery of GM-CSF for serum samples is 102.82%.
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프론티어 진행
의약품 개발 진행- 영어 이름：
Macrophage colony stimulating factor 2
- 카테고리：
- 상장 의약품 수：
0 상세
- 임상 의약품 수：
9 상세
- 최고 개발 단계：
NDA/BLA
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