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Product Overview
Human Complement C5a ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural Human Complement C5a that is present in biological samples, such as serum, plasma, and cell culture supernatants.It has no cross-reactivity with native human Complement C5 protein. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteComplement C5aFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity60 pg/mlAssay Time2.75 hrSample volume50 μLRange78.125 pg/mL-5000 pg/mLSample TypeFor the quantitative determination of human Complement C5a in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural human Complement C5a in serum, plasma and cell culture supernatants. It has no cross-reactivity with native human Complement C5 protein.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA261-C01Pre-coated Anti-Complement C5a Antibody Microplate1 plateCEA261-C02Human Complement C5a Standard20 μg×2CEA261-C03Biotin-Anti-Complement C5a Antibody Con. Solution300 μLCEA261-C04Biotin-Antibody Dilution Buffer8 mLCEA261-C05Streptavidin-HRP Con. Solution500 μLCEA261-C06Streptavidin-HRP Dilution Buffer15 mLCEA261-C0720× Washing Buffer50 mLCEA261-C08Sample Dilution Buffer15 mL×2CEA261-C09Substrate Solution12 mLCEA261-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Complement C5a is less than 60 pg/mL.
Sample Values
Healthy donor serum samples (n=32) were evaluated for the concentrations of human Complement C5a in the assay. The detectability rate of the samples was 100%, with a mean detectable concentration of 5.776 ng/mL and a concentration range of 1.299 to 25.084 ng/mL.
Intra-Assay Statistics
Ten replicates of each of 3 samples containing different Complement C5a concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of Complement C5a were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant Complement C5a was spiked into 3 human serum samples, and then analyzed. The average recovery of Complement C5a for serum samples is 92.5%.
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프론티어 진행
의약품 개발 진행- 영어 이름：
Complement C5a
- 카테고리：
- 상장 의약품 수：
1 상세
- 임상 의약품 수：
2 상세
- 최고 개발 단계：
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