Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) (MALS verified)

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Cat. No. / Size
Price
Qty
VP0-MY2108-100ug
$400.00
VP0-MY2108-1mg (500ug X 2)
$2655.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    14E1

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    Virus

  • Immunogen

    Recombinant Coxsackievirus A16 (strain G-10) VP0 Protein is expressed from human 293 cells

  • Specificity

    Specifically recognizes Coxsackievirus A16 (strain G-10) VP0 Protein.

  • Application

    Application
    Recommended Usage
    Western Blot
    10-0.02 μg/mL
  • Cross Verification

    The antibody can be used for WB detection.

  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     VP0 (Coxsackievirus A16) ELISA

    Immobilized Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag (Cat. No. VP0-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) (Cat. No. VP0-MY2108) with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol
  • Western Blot

     VP0 (Coxsackievirus A16) WESTERN BLOT
  • Detection of Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) by Western Blot. Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) at 0.02 μg/mL dilution + Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag at 400 ng.<br>
    Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.<br>
    Predicted band size: 40-60 kDa 12% Bis-Tris Protein Gel.

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Background

Cox A16 infection is very common in China, and the pathogenicity of Cox A16 is significantly different from enterovirus 71. Cox A16 can induce the production of a variety of micribonucleic acid, which affects the conduction of inflammatory signaling pathway, leading to the occurrence of pathological damage. Cox A16 capsid protein VP1, as an important target protein, interacts with host cell receptors to provide virion attachment to target host cells.

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