Product Details
Source
Monoclonal Anti-Influenza Virus NA broadly Antibody, Human IgG1 (1A12) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
1A12
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
Virus
Immunogen
Recombinant Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein
Specificity
Specific recognition of Influenza B [Austria/1359417/2021] Neuraminidase (NA)Protein.
Application
ApplicationRecommended UsageWestern Blot10-0.02 μg/mLCross Verification
This product can cross in Elisa with
Influenza A virus (A/Thailand/8/2022) NA(H3N2) Protein, His Tag (Cat. No. NEE-V524w).
Influenza A virus (Croatia/10136RV) & (Columbia/27) NA(H3N2) Protein, His Tag (Cat. No. NEE-V524x).
Influenza A [A/Victoria/4897/2022(H1N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524c).
Influenza A [Darwin/6/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5247).
Influenza A [A/Darwin/9/2021 (H3N2)] Neuraminidase (NA) Protein, His Tag (Cat. No. NE2-V5249).
Influenza A virus (A/Massachusetts/18/2022) NA(H3N2) Protein, His Tag (Cat. No. NEE-V524v).
Influenza A [Wisconsin/67/2022(H1N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524m).
Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5245).
Influenza B [PHUKET/3073/2013] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5246).
Influenza A [Victoria/2570/2019(H1N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524e).
Influenza A [Sydney/5/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5248).
Influenza A [turkey/Germany-MV/R2472/2014(H5N8)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5249).
Influenza A [A/Thailand/1(KAN-1)/2004(H5N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. HA1-V5245).
Influenza A [Guangdong/18SF020(H5N6)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524h).
Influenza B (B/Singapore/INFTT-16-0610/2016) Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524r).
Influenza B (B/Singapore/WUH4618/2021) Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524u).
Influenza A (A/Georgia/12/2022 CVR-167) NA (A/pdm09 linage) Protein, His Tag (Cat. No. NEE-V524p).
Influenza A (A/Sydney/1304/2022) Neuraminidase (NA) Protein, His Tag (Cat. No. NE2-V5247).Purification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA

Immobilized Influenza A [Wisconsin/588/2019(H1N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524k) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza Virus NA broadly Antibody, Human IgG1 (1A12) (Cat. No. NEE-MY2256) with a linear range of 0.05-1 ng/mL (QC tested).
Protocol
Western Blot

Detection of Monoclonal Anti-Influenza Virus NA broadly Antibody, Human IgG1 (1A12) by Western Blot. Monoclonal Anti-Influenza Virus NA broadly Antibody, Human IgG1 (1A12) at 0.02 μg/mL dilution + Influenza A [Wisconsin/67/2022(H1N1)] Neuraminidase (NA) Protein, His Tag at 400 ng.<br>
Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.<br>
Predicted band size: 55|65| 80-95 kDa 12% Bis-Tris Protein Gel
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