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제품 세부정보
Background
Recombinant Monoclonal Anti-Ki-67 Antibody, Rabbit (7A4) is a Ki-67 primary antibody for IHC application. Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.
Product Specifications
Host SpeciesRabbitIsotypeRabbit IgG, Rabbit KappaClone7A4ApplicationIHC-PDilution Ratio1: 200 - 1: 1000FormLiquidClonalityMonoclonalReactivityHumanSynonymsPPP1R105, MIB-1, ki-67Concentration0.9~1.1 mg/mlSpecificityReact with Ki-67 protein on the intracellular area of target cellsPurification TechProtein APreservationTris buffer, 1% BSAExperiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot. The shelf life is 30 days from the date of opening.ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
Sample Staining Data
Immunohistochemical analysis of paraffin embedded human cancer tissue labelled with HGS-S239 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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