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Product Details
Source
Monoclonal Anti-Human parainfluenza viruses Pre-fusion glycoprotein F0 Antibody, Human IgG1 (15A10) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
15A10
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
Virus
Immunogen
Recombinant Human parainfluenza viruses Pre-fusion glycoprotein F0 is expressed from human 293 cells
Specificity
Specifically recognizes Human parainfluenza viruses Pre-fusion glycoprotein F0.
Application
ApplicationRecommended UsageWestern Blot10-0.1 μg/mLPurification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Immobilized Human parainfluenza viruses Pre-fusion glycoprotein F0, His Tag (Cat. No. PR0-H52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human parainfluenza viruses Pre-fusion glycoprotein F0 Antibody, Human IgG1 (15A10) (Cat. No. HPV-MY2325) with a linear range of 0.04-2 ng/mL (QC tested).
Protocol
Western Blot
Detection of Monoclonal Anti-Human parainfluenza viruses Pre-fusion glycoprotein F0 Antibody, Human IgG1 (15A10) (MALS verified) by Western Blot. Monoclonal Anti-Human parainfluenza viruses Pre-fusion glycoprotein F0 Antibody, Human IgG1 (15A10) (MALS verified) at 0.10 μg/mL dilution + Human parainfluenza viruses Pre-fusion glycoprotein F0, His Tag (MALS verified) at 400 ng.<br>
Secondary: (HFC)-Peroxidase AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1/2000 dilution.<br>
Predicted band size: 65 kDa 12% Bis-Tris Protein Gel
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FAQ
- Product
What do “pre” and “post” refer to in the names of viral proteins?
In virology, “pre” refers to the conformation of a viral surface glycoprotein before fusion with the host cell membrane, while “post” refers to the conformation after membrane fusion and entry into the host cell. Both are naturally occurring states in the viral life cycle. Because the “pre” conformation represents a critical stage before viral entry, antibodies designed against this state often exhibit stronger neutralizing activity and are therefore more effective in preventing infection.
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