Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) (MALS verified)

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Cat. No. / Size
Price
Qty
GHL-MY2274-100ug
$350.00
GHL-MY2274-1mg (500ug X 2)
$2310.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    4D10

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    virus

  • Immunogen

    Recombinant HSV-1 (strain 17) gH&gL Protein is expressed from human 293 cells

  • Specificity

    Specifically recognizes HSV-1 (strain 17) gH&gL Protein.

  • Application

    Application
    Recommended Usage
    Western Blot
    0.02 μg/mL
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Glycoprotein H & Glycoprotein L/gH & gL (HSV) ELISA

    Immobilized HSV-1 (strain 17) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-H5283) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) (Cat. No. GHL-MY2274) with a linear range of 0.5-4 ng/mL (QC tested).

    Protocol
  • Western Blot

     Glycoprotein H & Glycoprotein L/gH & gL (HSV) WESTERN BLOT
  • Detection of Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) (MALS verified) by Western Blot Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) (MALS verified) at 0.02 μg/mL dilution + HSV-1 (strain 17) gH&gL Protein, Twin-Strep Tag&His Tag (MALS verified) at 400 ng.<br>
    Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) 1/2000 dilution.<br>
    Predicted band size: 95 kDa 12% Bis-Tris Protein Gel

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Background

The herpes simplex virus type 1 (HSV-1) gH-gL complex which is found in the virion envelope is essential for virus infectivity and is a major antigen for the host immune system.The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis.

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