Product Details
Source
Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (5G9) is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
5G9
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
Virus
Immunogen
Recombinant Nipah virus Glycoprotein G is expressed from human 293 cells
Specificity
Specifically recognizes Nipah virus Glycoprotein G.
Application
ApplicationRecommended UsageWestern Blot10-2 μg/mLPurification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA

Immobilized Nipah virus Glycoprotein G, His Tag (Cat. No. GLN-N52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (5G9) (Cat. No. GLN-MY2257) with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Western Blot

Detection of Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (5G9) by Western Blot. Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (5G9) at 2 μg/mL dilution + Nipah virus Glycoprotein G, His Tag (MALS verified) at 400 ng.
Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution
Predicted band size: 80-100 kDa 12% Bis-Tris Protein Gel
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