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PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 is a neutralizing antibody produced via site-specific conjugation of PE to Monoclonal Anti-FMC63 Antibody, Mouse IgG1 under optimal conditions with a proprietary technology.
We carry another premium grade PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (FM3-PY54G0), produced with the same production process but under more rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. It is designed for cell isolation and cell culture applications in the early preclinical stage.
Clone
Y45
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Recombinant FMC63 scFv derived from HEK293 cells
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Specificity
Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
Application
Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression). Please note that this product is NOT compatible to streptavidin detection system.
Recommended Dilution
1:50
Isotype Control
The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.5% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution;
- 2-8°C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Bioactivity-FACS
5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-PY54A2) and isotype control antibody respectively. PE signal was used to evaluate the binding activity (QC tested).
Protocol
Binding activity of the PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 before and after adding 0.03% Proclin was evaluated in the above FACS analysis. The result shows that PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-PY54A2) and PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) have the same binding activity against anti-CD19 CAR-293 cells.
Protocol
Non-specificity of PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-PY54A2) binding to CD3+ cells present in human PBMC. Human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
FACS Analysis of Blood Samples
After Fc receptor blockade, whole blood only control (Figure A) and whole blood spiked with CAR-T cells (Figure B) were first stained with PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (0.03% Proclin) (Cat. FM3-PY54A2), following 7-AAD staining. The data were analyzed using FCS Express 7 software (Routinely tested). CAR-T cells were identified as PE-positive events within the live CD45+CD3+ (or CD45+) lymphocyte population. The proportion of CD45+CAR+ cells is around 20%, which is largely consistent with the theoretical spike-in value.
Protocol
Evaluation of CAR expression
FACS Analysis of Non-specific binding to Human whole blood
Non-specificity of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Site-specific conjugation) (0.03% Proclin) (Cat. No. FM3-PY54A2) binding to CD3+ cells present in human whole blood. 100 μl of human whole blood were simultaneously stained with APC/Cyanine7 anti-human CD3 Antibody and PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (2 μL of the antibody stock solution in a final volume of 100 µL), compared with isotype control antibody and positive control antibody. Both APC/Cyanine7 and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells.
사용자 리뷰 댓글 남기기
G***o2025-11-06
좋아요The FM3-PY54A2 PE antibody from AcroBiosystems has proven to be an outstanding reagent for flow cytometry applications. It delivers exceptional signal clarity and consistency, providing optimal staining and reproducible results across multiple experimental runs. The antibody exhibits high specificity and minimal background, ensuring precise detection and reliable data quality. Overall, this product demonstrates excellent performance and stability, making it a highly recommended choice for researchers seeking robust and accurate flow cytometry analysis.
- M***i2025-06-13 좋아요This antibody demonstrates impressive stability and reliable detection, making it a valuable tool in the lab. Although it is somewhat expensive, its performance matches its cost. It works effectively across a wide range of staining conditions and has shown consistency across multiple cell types. Additionally, I have found that different lots are consistent. Overall, this is an excellent and dependable reagent.
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