PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (0.03% Proclin)

Flow cytometric analysis of Anti-BCMA (VHH format) CAR stained with PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-PMY2491a) at a 1:50 dilution (2 μL of antibody stock solution was used to stain 1 × 10⁶ cells in a final volume of 100 μL), with an isotype control antibody included as a negative control. PE fluorescence signals was used to evaluate binding activity (QC tested).

Flow cytometric analysis of the non-specific binding of PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-PMY2491a) to CD3+ cells in human PBMCs. A total of 5 × 10⁵ human PBMCs were stained with FITC-labeled anti-CD3 antibody alone (A) or co-stained with FITC-labeled anti-CD3 antibody and PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (B) (2 μL of antibody stock solution per 5 × 10⁵ cells in a final staining volume of 100 μL), washed, and analyzed by FACS. FITC and PE fluorescence signals were used to evaluate the non-specific binding of the PE-labeled antibody to human CD3+ cells (QC tested).

After Fc receptor blockade, the whole blood only control (Figure A) and the whole blood spiked with CAR-T cells (Figures B, C, and D) were first stained with anti-CD45 antibody, anti-CD3 antibody, and PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (0.03% Proclin) (Cat. CAH-PMY2491a), followed by 7-AAD staining. The data were analyzed using FCS Express 7 software (routinely tested). CAR-T cells were identified as PE-positive events within the live CD45⁺CD3⁺ (or CD45⁺) lymphocyte population. In panels B, C, and D, the proportions of CD45⁺ CAR⁺ cells were approximately 20%, 10%, and 1%, respectively, consistent with the theoretical spike in values of 22.39%, 11.49%, and 1.15%.

Binding activity of PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-PMY2491a) to different Humanized VHHs, as determined by ELISA. Humanized VHHs were coated at 1 μg/mL. The primary antibody (Cat. No. CAH-PMY2491a) was serially diluted starting from 125 ng/mL, and Peroxidase AffiniPure Goat Anti-Rabbit IgG, Fc Fragment Specific was used as the secondary antibody at a dilution of 1:30,000. Binding was measured by OD₄₅₀ (Routinely tested).

Binding activity of PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-PMY2491a) to different Alpaca VHHs, as determined by ELISA. Alpaca VHHs were coated at 1 μg/mL. The primary antibody (Cat. No. CAH-PMY2491a) was serially diluted starting from 62.5 ng/mL, and Peroxidase AffiniPure Goat Anti-Rabbit IgG, Fc Fragment Specific was used as the secondary antibody at a dilution of 1:30,000. Binding was measured by OD₄₅₀ (Routinely tested).