Cutting-Edge TR-FRET Solutions
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TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) is a detection technology that combines Fluorescence Resonance Energy Transfer (FRET) with Time-Resolved Fluorescence (TRF) measurement. It relies on the distance between donor and acceptor fluorophores—when they are brought close together, energy transfer occurs, generating a measurable signal. The time-resolved component introduces a delay in signal detection, effectively minimizing interference from autofluorescence and scattered excitation light.
By integrating the strengths of both FRET and TRF, TR-FRET offers high sensitivity, ease of use, and reliable results. It is well-suited for high-throughput screening and widely applicable in protein quantification, signaling pathway analysis, disease mechanism studies, and more—making it a valuable tool in modern drug discovery and development.
TR-FRET Principle
Fluorescence Resonance Energy Transfer (FRET): When molecules are not bound, the donor and acceptor remain far apart—typically more than 10 nm—so the excited donor cannot effectively transfer energy to the acceptor. As a result, only the donor emission peak (620 nm) is observed; When molecular binding occurs, the donor and acceptor are brought into close distance—usually less than 10 nm—allowing energy transfer from the excited donor to the acceptor via FRET. This results in emission from the acceptor, producing a detectable signal at 665 nm.
Time-Resolved Fluorescence (TRF) Detection: After the excitation pulse ends, fluorescence signals from the donor and acceptor are measured following a short delay—typically between 50 and 150 microseconds. This delay effectively eliminates interference from nonspecific background fluorescence. By analyzing the ratio of fluorescence intensities, TRF enables accurate assessment of molecular interactions, including binding strength, affinity, and kinetics.
Technology Advantages
High Throughput– Homogeneous assay with no washing steps
High Sensitivity– High signal-to-noise ratio with minimal background interference
Low Sample Consumption– Small reaction volumes save both samples and reagents
Excellent Reproducibility– Simple workflow minimizes experimental variability
Cutting-Edge TR-FRET Solutions
Leveraging its deep expertise in protein and antibody development, ACROBiosystems has successfully built a high-performance TR-FRET technology platform, delivering one-stop, cutting-edge solutions for biopharmaceutical research. We offer TR-FRET kits for diverse applications, highly compatible universal tools, and customized development services to help clients rapidly establish sensitive and reliable detection systems. Our solutions significantly enhance experimental efficiency—from target validation to preclinical studies—accelerating the development of innovative therapeutics.
High-Quality Core Raw Materials for Accurate Data
Leveraging mature protein and antibody platforms, ACRO selects raw materials with native structures, intact conformations, high biological activity, and excellent stability for TR-FRET product development. Through rigorous screening and validation, these materials ensure superior data accuracy and broad project compatibility.
Comprehensive and Rigorous Validation System
Prior to commercialization, each TR-FRET product undergoes systematic performance evaluation and validation in real-application validation, including multiple replicates and validation with marketed or clinical-stage drugs, ensuring high reliability and applicability.
Stable and Reliable Quality
From raw material selection and process development to final product release, ACRO implements end-to-end quality control. Multiple criteria are established to ensure stable performance and excellent batch-to-batch consistency.
Expanded Product Portfolio Compatible with Automated, High-Efficiency Assays
Covering major biopharmaceutical research areas, ACRO’s expanding products meet diverse needs of innovative R&D pipelines, and they are compatible with automated platforms, enabling scalable, high-throughput assays and significantly improving efficiency.
Flexible Purchasing & Custom Solutions
To meet diverse experimental needs, you can choose ready-to-use assay kits or flexibly Toolbox and proteins. TR-FRET Kit Development Service are also available, providing a one-stop solution.
Product Portfolio
- Protein-Protein Interaction Kit (PPI)
- Protein Quantification Kit
- Toolbox
Protein-Protein Interaction Kit (PPI)
Inhibition of Europium-chelate labeled human TSLP: FA labeled human IL-7 R alpha & TSLP R binding by Anti-TSLP Neutralizing Antibody Premix serial dilutions of Anti-TSLP Neutralizing Antibody (1:2 serial dilution, from 8 μg/mL to 0.0005 μg/mL (53.33-0.0033 nM)) and Human TSLP Protein Europium-chelate and incubate at room temperature (20℃-25℃) for 0.5 hours. Then add FA Labeled Human IL-7 R alpha & TSLP R and incubate at room temperature (20℃-25℃) for 0.5 hours. Detection was performed with IC50 of 0.2264nM. The assay was performed according to the above-described Datasheet (QC tested).
| Category | Cat. No. | Product Description |
|---|---|---|
| FcRn binding | FRT-01 | Human FcRn binding Kit (TR-FRET) |
| ADCC/ADCP Functional Evaluation | FRT-03 | Human Fc gamma RI / CD64 binding Kit (TR-FRET) |
| FRT-04 | Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) | |
| FRT-05 | Human Fc gamma RIIA / CD32a (R167) binding Kit (TR-FRET) | |
| FRT-06 | Human Fc gamma RIIIA / CD16a (F176) binding Kit (TR-FRET) | |
| FRT-07 | Human Fc gamma RIIIA / CD16a (V176) binding Kit (TR-FRET) | |
| Inhibitor Screening | FRT-02 | Human TL1A-DR3 inhibition Kit (TR-FRET) |
| FRT-P031 | Human TL1A / DcR3 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P011 | Human IL-17A / IL-17RA Inhibitor Screening Kit (TR-FRET) | |
| FRT-P009 | Human IL-4 / IL-4RA Inhibitor Screening Kit (TR-FRET) | |
| FRT-P012 | Human TSLP / IL-7Rα & TSLP R Inhibitor Screening Kit (TR-FRET) | |
| FRT-P008 | Human Fc epsilon RI alpha / IgE Fc Inhibitors Screening Kit (TR-FRET) | |
| FRT-P010 | Human IL-23 / IL-23R Inhibitor Screening Kit (TR-FRET) | |
| FRT-P014 | Human OX40 / OX40 Ligand Inhibitor Screening Kit (TR-FRET) | |
| FRT-P017 | Human IL-5 / IL-5Rα Inhibitor Screening Kit (TR-FRET) | |
| FRT-P018 | Human CD40 / CD40 Ligand Inhibitor Screening Kit (TR-FRET) | |
| FRT-P019 | Human PD-1 / PD-L1 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P022 | Human TNF-alpha / TNFR1 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P028 | Human TNF-alpha / TNFR2 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P023 | Human IL-6/ IL-6R Inhibitor Screening Kit (TR-FRET) | |
| FRT-P024 | Human VEGFA / VEGFR2 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P032 | Human Transferrin / Transferrin R Inhibitor Screening Kit (TR-FRET) | |
| Antibody Screening | FRT-P016 | Hybridoma Screening Kit (His-Antigen) (TR-FRET) |
Protein Quantification Kit
The kit demonstrates excellent linearity (R² = 1.0000) in complex matrix, confirming its suitability for real biological sample detection.
| Category | Cat. No. | Product Description |
|---|---|---|
| Fc Detection | FRT-Q015 | Human Fc Detection Kit (TR-FRET) |
| FRT-Q034 | High Performance Human Fc Detection Kit (TR-FRET) | |
| Cytokine Detection | FRT-C01 | Human IFN-gamma Detection Kit (TR-FRET) |
| FRT-C02 | Human Tumor Necrosis Factor Alpha (TNF-α) Detection Kit (TR-FRET) | |
| FRT-C03 | Human IL-2 Detection Kit (TR-FRET) |
Toolbox
In the TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled (Cat. No. FRT-TA04) is used as the Acceptor. An inhibition Assay was performed to evaluate the interaction between Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) and Human PD-L1, His Tag (Cat. No. PD1-H5229) using Monoclonal Anti-Human PD-1 Antibody, Human IgG4 resulting in a typical IC50 of 2.285 μg/mL.
| Category | Cat. No. | Product Description |
|---|---|---|
| Donor | FRT-TD09 | Streptavidin Protein Europium chelate (for TR-FRET) |
| FRT-TD02 | Monoclonal Anti-Human IgG Antibody Europium chelate (for TR-FRET) | |
| FRT-TD03 | Monoclonal Anti-His Tag Antibody Europium chelate (for TR-FRET) | |
| FRT-TD05 | Monoclonal Anti-Mouse IgG Antibody Europium chelate (for TR-FRET) | |
| FRT-TD06 | Monoclonal Anti-Flag Tag Antibody Europium chelate (for TR-FRET) | |
| Acceptor | FRT-TA01 | Streptavidin Protein FA-Labeled (for TR-FRET) |
| FRT-TA02 | Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) | |
| FRT-TA03 | Monoclonal Anti-Mouse IgG Antibody FA-Labeled (for TR-FRET) | |
| FRT-TA04 | Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled (for TR-FRET) | |
| FRT-TA05 | Monoclonal Anti-His Tag Antibody, Mouse IgG1 FA-Labeled (for TR-FRET) | |
| FRT-TA06 | Monoclonal Anti-Flag Tag Antibody FA-Labeled (for TR-FRET) | |
| FRT-TA11 | Monoclonal Anti-His Tag Antibody, Rabbit IgG FA-Labeled (for TR-FRET) | |
| Buffer | DB-04 | TR-FRET Sample Dilution Buffer, pH7.4 |
| DB-05 | TR-FRET Detection Buffer, pH7.4 | |
| DB-06 | TR-FRET Detection Buffer, for pH6.0 reaction system | |
| DB-07 | TR-FRET Detection Buffer, for pH5.5 reaction system | |
| DB-08 | TR-FRET Detection Buffer, for pH5.0 reaction system |
- Protein-Protein Interaction Kit (PPI)
- Protein Quantification Kit
- Toolbox
Protein-Protein Interaction Kit (PPI)
Inhibition of Europium-chelate labeled human TSLP: FA labeled human IL-7 R alpha & TSLP R binding by Anti-TSLP Neutralizing Antibody Premix serial dilutions of Anti-TSLP Neutralizing Antibody (1:2 serial dilution, from 8 μg/mL to 0.0005 μg/mL (53.33-0.0033 nM)) and Human TSLP Protein Europium-chelate and incubate at room temperature (20℃-25℃) for 0.5 hours. Then add FA Labeled Human IL-7 R alpha & TSLP R and incubate at room temperature (20℃-25℃) for 0.5 hours. Detection was performed with IC50 of 0.2264nM. The assay was performed according to the above-described Datasheet (QC tested).
| Category | Cat. No. | Product Description |
|---|---|---|
| FcRn binding | FRT-01 | Human FcRn binding Kit (TR-FRET) |
| ADCC/ADCP Functional Evaluation | FRT-03 | Human Fc gamma RI / CD64 binding Kit (TR-FRET) |
| FRT-04 | Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) | |
| FRT-05 | Human Fc gamma RIIA / CD32a (R167) binding Kit (TR-FRET) | |
| FRT-06 | Human Fc gamma RIIIA / CD16a (F176) binding Kit (TR-FRET) | |
| FRT-07 | Human Fc gamma RIIIA / CD16a (V176) binding Kit (TR-FRET) | |
| Inhibitor Screening | FRT-02 | Human TL1A-DR3 inhibition Kit (TR-FRET) |
| FRT-P031 | Human TL1A / DcR3 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P011 | Human IL-17A / IL-17RA Inhibitor Screening Kit (TR-FRET) | |
| FRT-P009 | Human IL-4 / IL-4RA Inhibitor Screening Kit (TR-FRET) | |
| FRT-P012 | Human TSLP / IL-7Rα & TSLP R Inhibitor Screening Kit (TR-FRET) | |
| FRT-P008 | Human Fc epsilon RI alpha / IgE Fc Inhibitors Screening Kit (TR-FRET) | |
| FRT-P010 | Human IL-23 / IL-23R Inhibitor Screening Kit (TR-FRET) | |
| FRT-P014 | Human OX40 / OX40 Ligand Inhibitor Screening Kit (TR-FRET) | |
| FRT-P017 | Human IL-5 / IL-5Rα Inhibitor Screening Kit (TR-FRET) | |
| FRT-P018 | Human CD40 / CD40 Ligand Inhibitor Screening Kit (TR-FRET) | |
| FRT-P019 | Human PD-1 / PD-L1 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P022 | Human TNF-alpha / TNFR1 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P028 | Human TNF-alpha / TNFR2 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P023 | Human IL-6/ IL-6R Inhibitor Screening Kit (TR-FRET) | |
| FRT-P024 | Human VEGFA / VEGFR2 Inhibitor Screening Kit (TR-FRET) | |
| FRT-P032 | Human Transferrin / Transferrin R Inhibitor Screening Kit (TR-FRET) | |
| Antibody Screening | FRT-P016 | Hybridoma Screening Kit (His-Antigen) (TR-FRET) |
TR-FRET Product Inquiry
Need support with TR-FRET assay development or product selection?
Submit your inquiry to connect with our technical team. We’ll provide guidance, product recommendations, and experimental insights tailored to your application.
TR-FRET Toolbox Free Sample Campaign
Campaign Period: February 6, 2026 – June 30, 2026
Get a Dr. Llama Charm with Any TR-FRET Purchase
TR-FRET Kit Development Service
In response to the growing complexity of biopharmaceutical R&D, ACROBiosystems now offers specialized custom TR-FRET kit development service. Our tailored solutions empower your research with:
Enhanced Performance– High sensitivity and reproducibility to streamline your workflow
Low Sample Consumption– Small reaction volumes save both samples and reagents
End-to-End Support– From initial design to validated kit delivery
Whether you are developing small molecule drugs or biologics, our TR-FRET solutions provides the precision tools you need to shorten development timelines and improve data quality!
Service Workflow
| Service Stage | Deliverables | Timeline | Price |
|---|---|---|---|
| Method Development |
1. Method development data （Note: Method development data provided only.） |
2-3 weeks |
Project-based, customized quotation. Contact us for a tailored plan. |
| Method Validation | 1. Method validation report | 2-3 weeks | |
| Kit Production |
1. TR-FRET kit/components 2. Quality inspection report (COA) 3. Detailed user manual (DS) |
2-3 weeks |
Resources
TR-FRET Campaign—Lunch&Learn
- Background
- TR-FRET Principle
- Technology Advantages
- Cutting-Edge TR-FRET Solutions
- Product Portfolio
- TR-FRET Product Inquiry
- TR-FRET Toolbox Free Sample Campaign
- Get a Dr. Llama Charm with Any TR-FRET Purchase
- TR-FRET Kit Development Service
- Resources
온라인 메시지
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Email：order@acrobiosystems.com
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