Human Tumor Necrosis Factor Alpha (TNF-α) Detection Kit (TR-FRET)

Cat. No. / Size
Price
Qty
FRT-C02-100tests
$450.00
FRT-C02-500tests
$780.00
ETA of in-stock products:2 business days

Product Details

  • FAB

    1. Cost effective - Sufficient quantity at a lower price, accounting for dilution and pipetting losses.
    2. Comprehensive validation - Validated with various antibody subtypes and antibody drugs.
    3. Simple and fast operation - No complicated washing steps, significantly reducing time.
    4. High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
    5. Accurate and reliable results - High sensitivity with minimal matrix effects.
    6. High throughput capability - Supports 500 tests, ideal for high-throughput screening.
    7. Fast completion - Results in just 2 hour.
  • Product Overview

    The Human Tumor Necrosis Factor Alpha (TNF-α) Detection kit (TR-FRET) can detect the Human Tumor Necrosis Factor Alpha (TNF-α) in homogeneous system within 2 hours, it is highly sensitive, short detection time and easy to use. This kit is specifically designed for the accurate quantitation of human TNF-alpha from cell culture supernatant, serum and plasma.

  • Product Specifications

    Assay Type
    Sandwich-TR-FRET
    Analyte
    Human TNF-α
    Reactivity
    Human
    Regulatory Status
    RUO
    Sensitivity
    LOQ=39 pg/mL
    Standard Curve Range
    39 pg/mL-2500 pg/mL
    Assay Time
    2 hr
    Suitable Sample Type
    For human TNF-α detection in human serum, human plasma,cell culture supernatants
    Sample volume
    16 μL

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Workflow

    TNF-alpha Workflow

  • Storage

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    FRTC02-C01
    Anti-TNF-α Antibody Europium-chelate
    100 tests/500 tests
    FRTC02-C02
    FA Labeled Anti-TNF-α Antibody
    100 tests/500 tests
    FRTC02-C03
    Human TNF-α Standard
    15 μg/100 tests & 500 tests
    DB-04
    TR-FRET Sample Dilution Buffer, pH7.4
    50 mL/100 tests & 500 tests
    DB-05
    TR-FRET Detection Buffer, pH7.4
    50 mL/100 tests & 500 tests
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Typical Data 

    Please refer to DS document for the assay protocol.

     TNF-alpha TYPICAL DATA

    Standard curve generated in TR-FRET Sample Dilution Buffer, pH7.4 (DB-04):

Validation
  • Dilution Linearity

    To assess the linearity of the assay, samples spiked with high concentrations of human TNF-α were serially diluted with TR-FRET Sample Dilution Buffer, pH7.4 (DB-04) to produce samples with values within the dynamic range of the assay.

     TNF-alpha DILUTION LINEARITY
  • Intra-Assay Statistics

    Five Samples of known concentrations were tested repeatedly 16 times in batches to assess within-batch precision and accuracy.

     TNF-alpha INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Five Samples of known concentrations were tested repeatedly across three independent analytical batches to evaluate between-batch precision and accuracy.

     TNF-alpha INTER-ASSAY STATISTICS

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Recent Advances

 
Drug Development Progress
  • English Name:

    Tumor necrosis factor α

  • Category:

  • Approved Drugs:

    68 Details

  • Drugs in Clinical Trials:

    74 Details

  • Highest Development Stage:

    Approved

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