Product Details
FAB
- Cost effective - Sufficient quantity at a lower price, accounting for dilution and pipetting losses.
- Comprehensive validation - Validated with various antibody subtypes and antibody drugs.
- Simple and fast operation - No complicated washing steps, significantly reducing time.
- High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
- Accurate and reliable results - High sensitivity with minimal matrix effects.
- High throughput capability - Supports 500 tests, ideal for high-throughput screening.
- Fast completion - Results in just 2 hour.
Product Overview
The Human Tumor Necrosis Factor Alpha (TNF-α) Detection kit (TR-FRET) can detect the Human Tumor Necrosis Factor Alpha (TNF-α) in homogeneous system within 2 hours, it is highly sensitive, short detection time and easy to use. This kit is specifically designed for the accurate quantitation of human TNF-alpha from cell culture supernatant, serum and plasma.
Product Specifications
Assay TypeSandwich-TR-FRETAnalyteHuman TNF-αReactivityHumanRegulatory StatusRUOSensitivityLOQ=39 pg/mLStandard Curve Range39 pg/mL-2500 pg/mLAssay Time2 hrSuitable Sample TypeFor human TNF-α detection in human serum, human plasma,cell culture supernatantsSample volume16 μLElevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Workflow

Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeFRTC02-C01Anti-TNF-α Antibody Europium-chelate100 tests/500 testsFRTC02-C02FA Labeled Anti-TNF-α Antibody100 tests/500 testsFRTC02-C03Human TNF-α Standard15 μg/100 tests & 500 testsDB-04TR-FRET Sample Dilution Buffer, pH7.450 mL/100 tests & 500 testsDB-05TR-FRET Detection Buffer, pH7.450 mL/100 tests & 500 testsACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.

Standard curve generated in TR-FRET Sample Dilution Buffer, pH7.4 (DB-04):
Dilution Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human TNF-α were serially diluted with TR-FRET Sample Dilution Buffer, pH7.4 (DB-04) to produce samples with values within the dynamic range of the assay.

Intra-Assay Statistics
Five Samples of known concentrations were tested repeatedly 16 times in batches to assess within-batch precision and accuracy.

Inter-Assay Statistics
Five Samples of known concentrations were tested repeatedly across three independent analytical batches to evaluate between-batch precision and accuracy.

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Recent Advances
- English Name:
Tumor necrosis factor α
- Category:
- Approved Drugs:
68 Details
- Drugs in Clinical Trials:
74 Details
- Highest Development Stage:
Approved
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