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- ProductSizeAmountActiveMax® Streptavidin μBeads, premium grade (for cells)10 mg1×10⁸ beadsActiveMax® Streptavidin μBeads, premium grade (for cells)100 mg (10 mg×10)1×10⁹ beads
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This product can be directly isolated using conventional column-free magnetic stand (eg. Cat. MB-01/ MB-02/ MB-03/ MB-04). Please note that due to its micron-scale size, it is not compatible with commercially available column-based (M/D/L) magnetic separation systems.
Product Description
ActiveMax® Streptavidin μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm with streptavidin on its surface. Streptavidin is a tetrameric protein providing 4 high-affinity biotin binding sites, which make it possible to fast prepare and customize your own sterile protein/antibody coupled beads for cell based assay, cell sorting, cell therapy process development and manufacturing use.
ActiveMax® Streptavidin μBeads, premium grade (for cells) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.Application
ActiveMax® Streptavidin coupled-μbeads, premium grade (for cells)can be used for coupling of any biotinylated proteins or antibodies, and are ideal for numerous applications as follows:
When coupled with biotinylated CAR target proteins, it can be used to stimulate or isolate CAR-T cells or UCAR-T cells.
When coupled with biotinylated antibodies, it can be used to isolate antigen specific cells, including immune cells, tumor cells, etc.
When coupled with biotinylated drug targets, it can be used for screening target specific antibodies by FACS.
It can also be used for other cell based experiments that you need.Reconstitution
See Certificate of Analysis (CoA) for detailed instruction.
Storage
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 6 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
Sterility
Negative
Endotoxin
Less than 0.002 EU per μg by the LAL method / rFC method.
Mycoplasma
Negative
Important Note
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
Formulation
Lyophilized in PBS with 0.1% HSA，pH 7.4. Trehalose is added as protectant before lyophilization.
ACRO Quality Management System
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Performance Data
FACS Data
<strong>ActiveMax Streptavidin coupled μbeads, premium grade coupled with protein.</strong> 40ug of the biotinylated human BCMA protein (Cat. No. BCA-H82E4) was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the coupled μbeads were fluorescently stained using PE labeled anti-human BCMA antibody and analyzed by flow cytometry.
Protocol
<strong>ActiveMax Streptavidin coupled μbeads, premium grade coupled with antibody.</strong> 50ug of the biotinylated anti-FMC63 Antibody (Cat. No. FM3-BY54) was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the coupled μbeads were fluorescently stained using PE labeled anti-mouse Fc antibody and analyzed by flow cytometry.
Protocol
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