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Application
The Monoclonal Anti-Human IgG Antibody Europium chelate is a universal tool for TR-FRET assays that can bind human IgG or hFc-tagged proteins. This product uses specific anti-human IgG antibody covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.Product Specifications
SourceThis Monoclonal Anti-Human IgG Antibody is a mouse monoclonal antibody recombinantly expressed from Hybridoma.SpecificityThis antibody recognizes all four human IgG isotypes (IgG1, IgG2, IgG3, and IgG4) and multiple engineered variants, including: Human IgG1 (N297A), Human IgG1 (L234A, L235A), Human IgG1 (M252Y, S254T, T256E), Human IgG1 (L234F, L235E, P331S), Human IgG1 (S239D, I332E), Human IgG1 (S298A, E333A, K334A), Human IgG1 (T307A, E380A, N434A), Human IgG2 (A330S, P331S), Human IgG2 (P331S), Human IgG4 (S108P), and Human IgG4 (S228P, L235E).Cross-ReactivityWeak cross-reactivity with cynomolgus IgG, whereas no reactivity to human IgM, human IgA, mouse IgG isotypes (IgG1, IgG2a, IgG2b) or rabbit IgG.FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.Physical AppearancePowderStorage (unopened)-20°C to -70°CStorage (opened)-70°CUnit Size1000 tests & 5000 tests & 20000 testsTechnologyTR-FRETIntended UseFor binding the human IgG antibody or human Fc tagged protein as donor in TR-FRET AssayReconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Performance Data
Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Monoclonal Anti-Human IgG Antibody Europium chelate (Cat. No. FRT-TD02) is used as the Donor and Streptavidin Protein FA-Labeled (Cat. No. FRT-TA01) is used as the Acceptor. Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4), at 16 ng/well, can bind to the Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a linear detection range of 10-0.02 μg/mL (QC tested).
In this TR-FRET assay, Monoclonal Anti-Human IgG Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD02) is used as the Donor and Monoclonal Anti-His Tag Antibody, Rabbit IgG FA-Labeled (for TR-FRET) (Cat. No. FRT-TA11) is used as the Acceptor. Human Her2, His Tag (Cat. No. HE2-H5225), at 1.25 ng/well, can bind to the Disitamab with a linear detection range of 2-0.002 μg/mL (Routinely tested).
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Drug Development Progress- English Name：
Immunoglobulin G
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Approved
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