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Product Overview
Human TPO ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TPO that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteTPOFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity<31.25 pg/mLAssay Time2.75 hrSample volume50 μLRange31.25 pg/mL-2000 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code03/124Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human TPO in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA201-C01Pre-coated Anti-TPO Antibody Microplate1 plate (8×12 strips)CEA201-C02Human TPO Standard16 μg×2CEA201-C03Biotin-Anti-TPO Antibody Con. Solution500 μLCEA201-C04Biotin-Antibody Dilution Buffer8 mLCEA201-C05Streptavidin-HRP Con. Solution500 μLCEA201-C06Streptavidin-HRP Dilution Buffer15 mLCEA201-C0720×Washing Buffer50 mLCEA201-C08Sample Dilution Buffer15 mL×2CEA201-C09Substrate Solution12 mLCEA201-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of TPO is less than 31.25 pg/mL.
Sample Values
Serum Sample: TPO concentrations were measured in eight human serum samples, which were collected from healthy human subjects. The measurements of eight samples are shown in the figure below.
Dilution Linearity
High concentrations of human TPO serum samples were diluted with 1:2, 1:4, 1:8, 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of TPO was 111.3%.
Intra-Assay Statistics
Ten replicates of each of four samples containing different TPO concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Five samples containing different concentrations of TPO were tested in the independent assays. Acceptable criteria: CV<15%.
Recovery
TPO was spiked into three human serum samples, and then analyzed. The average recovery of TPO for serum samples is 111.3%.
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