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Product Overview
Mouse TNF-alpha ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant mouse TNF-alpha that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteMouse TNF-alphaFormat96-wells plate breakable into 12 x 8 wells stripsReactivityMouseSensitivity4 pg/mLAssay Time2.75 hrSample volume50 μLRange7.81 pg/mL-500 pg/mLSample TypeFor the quantitative determination of mouse TNF-alpha in serum, plasma and cell supernatant.NIBSC Code88/532, NIBSC/WHO (88/532) approximate value (U/mL) = 0.2 x ACRO Mouse TNF-α (pg/mL)Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant mouseTNF-alpha in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA255-C01Pre-coated Anti-mouse TNF-alpha Antibody Microplate1 plateCEA255-C02Mouse TNF-alpha Standard5 ng×2CEA255-C03Biotin-Anti-mouse TNF-alpha Antibody Con. Solution100 μLCEA255-C04Biotin-Antibody Dilution Buffer8 mLCEA255-C05Streptavidin-HRP Con. Solution500 μLCEA255-C06Streptavidin-HRP Dilution Buffer15 mLCEA255-C0720×Washing Buffer50 mLCEA255-C08Sample Dilution Buffer15 mL×2CEA255-C09Substrate Solution12 mLCEA255-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of mouse TNF-alpha is less than 4 pg/mL.
Sample Values
RAW 264.7 mouse monocyte/macrophage cells (3.5 x 10⁶ cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 ug/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 5.0 ug/mL LPS and 100 ng/mL rmlL-10 for 5 and 7 days. Subsequent analysis involved quantitative measurement of mouse TNF-alpha concentrations in collected culture supernatants using standardized immunoassays.
Intra-Assay Statistics
Ten replicates of each of three samples containing different TNF-α concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of TNF-α were tested in in-dependent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant TNF-alpha was spiked into three mouse serum samples, and then analyzed. The average recovery of TNF-alpha for serum samples is 90.1%.
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프론티어 진행
의약품 개발 진행- 영어 이름：
Tumor necrosis factor α
- 카테고리：
- 상장 의약품 수：
68 상세
- 임상 의약품 수：
74 상세
- 최고 개발 단계：
Approved
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