제품 수집
제품 세부정보
Product Overview
Human TNF-αlpha Quick ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TNF-alpha that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteTNF-αFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity2 pg/mLRange19.5 pg/mL-1250 pg/mLAssay Time1.75 hrSample volume50 μLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code12/154Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human TNF-alpha in serum, plasma and cell culture supernatants.
It is suitable for potency assay of mAbs.
This Assay has been anchored to NlBSC/WHO standards (Code: 12/154), ELISA Kit: NIBSC/WHO Mass Conversion Factor is 1.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA246-C01Pre-coated Anti-TNF-α Antibody Microplate1 plate (8×12 strips)CEA246-C02Human TNF-α Standard12.5 ng×2CEA246-C03Biotin-Anti-TNF-α Antibody20 μgCEA246-C04Biotin-Antibody Dilution Buffer8 mLCEA246-C05Streptavidin-HRP Con. Solution500 μLCEA246-C06Streptavidin-HRP Dilution Buffer15 mLCEA246-C0720×Washing Buffer50 mLCEA246-C08Sample Dilution Buffer15 mL×2CEA246-C09Substrate Solution12 mLCEA246-C10Stop Solution6 mLACRO Quality Management System
고객님도 확인한 상품
데이터 표시
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of TNF-α is less than 2.0 pg/mL.
Sample Values
Cell Culture Supernatant: Human peripheral blood mononuclear cells (PBMCs) obtained were plated at a density of 1 × 10⁶ cells/mL in complete RPMI 1640 medium containing 10% fetal bovine serum, 2 mM L-glutamine, and antibiotic supplements (penicillin 100 U/mL with streptomycin sulfate 100 μg/mL). Following a 5-day incubation period, experimental groups were either maintained as untreated controls or stimulated with 10 μg/mL phytohemagglutinin (PHA). Subsequent analysis involved quantitative measurement of TNF-alpha concentrations in collected culture supernatants using standardized immunoassays.
Intra-Assay Statistics
Ten replicates of each of three samples containing different TNF-α concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of TNF-α were tested in inde-pendent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant TNF-α was spiked into three human serum samples, and then analyzed. The average recovery of TNF-α for serum samples is 91.3%.
사용자 리뷰 댓글 남기기
프론티어 진행
의약품 개발 진행- 영어 이름：
Tumor necrosis factor α
- 카테고리：
- 상장 의약품 수：
68 상세
- 임상 의약품 수：
74 상세
- 최고 개발 단계：
Approved
연락하기
전화
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521
신약 후보물질 라이선싱
