Human SCF ELISA Kit

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제품 번호/규격
가격
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CEA-C078-96tests
$515.00
CEA-C078-480tests (96tests X 5)
$2310.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Product Overview

    Human SCF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human SCF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    SCF
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    15.625 pg/mL
    Assay Time
    3 hr 15 min
    Sample volume
    50 μL
    Range
    31.25 pg/mL-2000 pg/Ml
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.
    NIBSC Code
    91/682

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant human SCF in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow

    SCF Workflow

  • Storage

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CEA078-C01
    Pre-coated Anti- SCF Antibody Microplate
    1 plate
    CEA078-C02
    Human SCF Standard
    20 μg×2
    CEA078-C03
    Biotin-Anti-SCF Antibody Con. Solution
    200 μL
    CEA078-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CEA078-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CEA078-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA078-C07
    20×Washing Buffer
    50 mL
    CEA078-C08
    Sample Dilution Buffer
    15 mL×2
    CEA078-C09
    Substrate Solution
    12 mL
    CEA078-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Typical Data 

    Please refer to DS document for the assay protocol.

     SCF TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of SCF is less than 15.625 pg/mL.

Validation
  • Sample Values

    Serum Sample: Eight healthy serum samples were evaluated for the concentrations of human SCF in assay.

     SCF SAMPLE VALUES
  • Intra-Assay Statistics

    Ten replicates of each of four samples containing different SCF concentrations were tested in one assay. Acceptable criteria: CV<10%.

     SCF INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Five samples containing different concentrations of SCF were tested in independent assays. Acceptable criteria: CV<15%.

     SCF INTER-ASSAY STATISTICS
  • Recovery

    Recombinant SCF was spiked into three human serum samples, and then analyzed. The average recovery of SCF for serum samples is 103.2%.

     SCF RECOVERY

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