Human soluble C5b-9 (sC5b-9) ELISA Kit

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CEA-B256-96tests
$790.00
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제품 세부정보

  • Product Overview

    Human soluble C5b-9 (sC5b-9) ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate human sC5b-9 that is present in biological samples, such as serum, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    2. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    3. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    soluble C5b-9 (sC5b-9)
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    1.5 ng/mL
    Assay Time
    3.25 hr
    Sample volume
    100 μL
    Range
    4.0 ng/mL-30 ng/mL
    Sample Type
    For the quantitative determination of human SC5b-9, plasma and cell supernatant.

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant human SC5b-9 in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow

    SC5b-9 Workflow

  • Storage

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CEA256-C01
    Pre-coated Anti-SC5b-9 Antibody Microplate
    1 plate
    CEA256-C02
    Human SC5b-9 Standard
    37.5 ng/mL×4
    CEA256-C03
    Biotin-Anti-SC5b-9 Antibody Con. Solution
    400 μL
    CEA256-C04
    Biotin-Antibody Dilution Buffer
    15 mL
    CEA256-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CEA256-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA256-C07
    20× Washing Buffer
    50 mL
    CEA256-C08
    Sample Dilution Buffer
    15 mL×2
    CEA256-C09
    Substrate Solution
    12 mL
    CEA256-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Typical Data 

    Please refer to DS document for the assay protocol.

     SC5b-9 TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Human SC5b-9 is less than 1.5 ng/mL.

Validation
  • Sample Values

    Healthy donor serum samples (n=56) were evaluated for the concentrations of human sC5b-9 in the assay. The detectability rate of the samples was 100%, with a mean detectable concentration of 1002.8 ng/mL and a concentration range of 696.9 to 1254.7 pg/mL.

     SC5b-9 SAMPLE VALUES
  • Intra-Assay Statistics

    Ten replicates of each of 3 samples containing different SC5b-9 concentrations were tested in one assay. Acceptable criteria: CV<10%.

     SC5b-9 INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Three samples containing different concentrations of SC5b-9 were tested in independent assays. Acceptable criteria: CV<15%.

     SC5b-9 INTER-ASSAY STATISTICS

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