Human Perforin ELISA Kit

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제품 번호/규격
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CEA-B034-96tests
$515.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Product Overview

    Human Perforin ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Perforin that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    Perforin
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    60 pg/mL
    Assay Time
    2.75 hr
    Sample volume
    100 μL
    Range
    62.5 pg/mL-2000 pg/mL
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant human Perforin in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow

    Perforin Workflow

  • Storage

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CEA034-C01
    Pre-coated Anti-Perforin Antibody Microplate
    1 plate
    CEA034-C02
    Human Perforin Standard
    1ng×2
    CEA034-C03
    Biotin-Anti-Perforin Antibody Con. Solution
    150 μL
    CEA034-C04
    Biotin-Antibody Dilution Buffer
    15 mL
    CEA034-C05
    Streptavidin-HRP Con. Solution
    1.5 mL
    CEA034-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA034-C07
    20×Washing Buffer
    50 mL
    CEA034-C08
    Sample Dilution Buffer
    15 mL×2
    CEA034-C09
    Substrate Solution
    12 mL
    CEA034-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Typical Data 

    Please refer to DS document for the assay protocol.

     Perforin TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Perforin is less than 60 pg/mL.

Validation
  • Intra-Assay Statistics

    Ten replicates of each of three samples containing different Perforin concentrations were tested in one assay. Acceptable criteria: CV<10%.

     Perforin INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Three samples containing different concentrations of Perforin were tested in independent assays. Acceptable criteria: CV<15%.

     Perforin INTER-ASSAY STATISTICS

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