Biotinylated Human PD-L2 / B7-DC Protein, Fc,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
PD2-H82F6-25ug
$360.00
PD2-H82F6-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    PDL2, PD-L2, Butyrophilin B7-DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2

  • Source

    Biotinylated Human PD-L2, Fc,Avitag (PD2-H82F6) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # Q9BQ51-1).

    Predicted N-terminus: Leu 20

    Request for sequence
  • Molecular Characterization

    PD-L2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 51.0 kDa. The protein migrates as 66-91 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    PD-L2 SDS-PAGE

    Biotinylated Human PD-L2, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    PD-L2 SEC-MALS

    The purity of Biotinylated Human PD-L2, Fc,Avitag (Cat. No. PD2-H82F6) is more than 90% and the molecular weight of this protein is around 120-140 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     PD-L2 ELISA

    Immobilized Human PD-1, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L2, Fc,Avitag (Cat. No. PD2-H82F6) with a linear range of 0.5-16 ng/mL (QC tested).

    Protocol

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Background

Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Recent Advances

 
Drug Development Progress
  • English Name:

    Programmed death-ligand 2

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    5 Details

  • Highest Development Stage:

    Phase 3 Clinical

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