제품 수집
제품 세부정보
Synonym
PDL2, PD-L2, Butyrophilin B7-DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2
Source
Human PD-L2 Protein, Fc Tag (PD2-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).
Predicted N-terminus: Leu 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
SDS-PAGE
Human PD-L2 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) is more than 90% and the molecular weight of this protein is around 115-140 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Cat PD-1 Protein, His,Avitag (Cat. No. PD1-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.2-1 ng/mL (Routinely tested).
Protocol
Immobilized Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.2-4 ng/mL (Routinely tested).
Protocol
Immobilized Human PD-L2, Fc Tag (Cat. No. PD2-H5251) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Mouse IgG2a Fc,Avitag (Cat. No. PD1-H82A4) with a linear range of 1-78 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human PD-1, His Tag (Cat. No. PD1-H5221) on HIS1K Biosensor, can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) on Protein A Biosensor, can bind Human PD-1, His Tag (Cat. No. PD1-H5221) with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
Flow Cytometry assay shows that recombinant Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 μg/mL (Routinely tested).
Protocol
Bioactivity-CELL BASE
Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with an EC50 of 0.018 μg/mL (Routinely tested).
Protocol
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배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Programmed death-ligand 2
- 카테고리：
- 상장 의약품 수：
0 상세
- 임상 의약품 수：
5 상세
- 최고 개발 단계：
Phase 3 Clinical
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전화
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