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Product Details
Synonym
PDCD1, PD1, CD279, SLEB2
Source
Biotinylated Canine PD-1, His,Avitag (PD1-C52E5) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gly 168 (Accession # NP_001301026.1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 19.7 kDa. The protein migrates as 32-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Canine PD-1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Canine PD-1, His,Avitag (Cat. No. PD1-C52E5) is more than 85% and the molecular weight of this protein is around 27-40 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at 2 μg/mL (100 μL/well) can bind Biotinylated Canine PD-1, His,Avitag (Cat. No. PD1-C52E5) with a linear range of 1-63 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Canine PD-1, His,Avitag (Cat. No. PD1-C52E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a linear range of 0.004-1 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed cell death protein 1
- Category：
- Approved Drugs：
22 Details
- Drugs in Clinical Trials：
193 Details
- Highest Development Stage：
Approved
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