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Product Overview
Human PCSK9 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human PCSK9 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalytePCSK9Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity0.45 ng/mLAssay Time2.75 hrSample volume50 μLRange0.625 ng/mL-20 ng/mLSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human PCSK9 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA202-C01Pre-coated Anti-PCSK9 Antibody Microplate1 plateCEA202-C02Human PCSK9 Standard20 μg×2CEA202-C03Biotin-Anti-PCSK9 Antibody Con. Solution100 μLCEA202-C04Biotin-Antibody Dilution Buffer8 mLCEA202-C05Streptavidin-HRP Con. Solution500 μLCEA202-C06Streptavidin-HRP Dilution Buffer15 mLCEA202-C0720×Washing Buffer50 mLCEA202-C08Sample Dilution Buffer15 mL×2CEA202-C09Substrate Solution12 mLCEA202-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of PCSK9 is less than 0.45 ng/mL.
Sample Values
Serum Sample: PCSK9 concentrations were measured in twenty-four human serum samples (dilution ratio 1:40), sample data shows in the figure below.
Dilution Linearity
High concentrations of human PCSK9 serum samples were diluted with 1:2, 1:4, 1:8 and 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of PCSK9 was 100.6%.
Intra-Assay Statistics
Ten replicates of each of three samples containing different PCSK9 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of PCSK9 were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant PCSK9 was spiked into three human serum samples, and then analyzed. The average recovery of PCSK9 for serum samples is 96.4%.
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프론티어 진행
의약품 개발 진행- 영어 이름：
Proprotein convertase subtilisin kexin type 9
- 카테고리：
- 상장 의약품 수：
8 상세
- 임상 의약품 수：
35 상세
- 최고 개발 단계：
Approved
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