제품 수집
제품 세부정보
Synonym
CD112, Nectin-2, PVRL2
Source
Human Nectin-2, His Tag (PV2-H52E2) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).
Predicted N-terminus: Gln 32
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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데이터 표시
SDS-PAGE
Human Nectin-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Nectin-2, His Tag (Cat. No. PV2-H52E2) is more than 95% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human PVRIG, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with a linear range of 0.078-5 μg/mL (QC tested).
Protocol
Immobilized Human PVRIG, Fc Tag (Cat. No. PVG-H5257) at 5 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with a linear range of 0.039-0.625 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human PVRIG, Fc Tag (Cat. No. PVG-H5257) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.61 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Rat TIGIT, Fc Tag (Cat. No. TIT-R5258) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.59 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.66 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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