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Product Overview
Human Soluble BCMA ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human BCMA that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteSoluble BCMAFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity150 pg/mLAssay Time2 hr 45 minSample volume50 μLRange375 pg/mL-24000 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human BCMA in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA045-C01Pre-coated Anti-Soluble BCMA Antibody Microplate1 plateCEA045-C02Human Soluble BCMA Standard320 μg×2CEA045-C03Biotin-Anti-Soluble BCMA Antibody Con. Solution100 μLCEA045-C04Biotin-Antibody Dilution Buffer8 mLCEA045-C05Streptavidin-HRP Con. Solution500 μLCEA045-C06Streptavidin-HRP Dilution Buffer15 mLCEA045-C0720×Washing Buffer50 mLCEA045-C08Sample Dilution Buffer15 mL×2CEA045-C09Substrate Solution12 mLCEA045-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of BCMA is less than 150 pg/mL.
Sample Values
Serum Sample: thirty healthy donor serum samples were evaluated for the concentrations of human Soluble BCMA in assay. The sample detection rate was 100%, with a range of 34.3-162.5 ng/mL and a mean of 77.7 ng/mL.
Intra-Assay Statistics
Ten replicates of each of three samples containing different BCMA concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of BCMA were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant BCMA was spiked into three human serum samples, and then analyzed. The average recovery of BCMA for serum samples is 97.4%.
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프론티어 진행
의약품 개발 진행- 영어 이름：
B-cell maturation protein
- 카테고리：
- 상장 의약품 수：
8 상세
- 임상 의약품 수：
145 상세
- 최고 개발 단계：
Approved
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