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Product Overview
Human Insulin ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human insulin that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteInsulinFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity4.69 μIU/mLAssay Time1.75 hrSample volume50 μLRange4.69 μIU/mL-300 μIU/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code11/212Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human insulin in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA205-C01Pre-coated Anti-InsμLin Antibody Microplate1 plateCEA205-C02Human InsμLin Standard (Std.-1)300 μIU/mLCEA205-C03Biotin-Anti-InsμLin Antibody Con. Solution400 μLCEA205-C04Biotin-Antibody Dilution Buffer8 mLCEA205-C05Streptavidin-HRP Con. Solution500 μLCEA205-C06Streptavidin-HRP Dilution Buffer15 mLCEA205-C0720×Washing Buffer50 mLCEA205-C08Sample Dilution Buffer15 mL×2CEA205-C09Substrate Solution12 mLCEA205-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Insulin is less than 4.69 μIU/mL.
Sample Values
Serum Sample: Insulin concentrations were measured in thirty-two human serum samples, which were collected from healthy human subjects. The measurements of thirty-two samples are shown in the figure below.
Dilution Linearity
High concentrations of human Insulin serum samples were diluted with 1:2, 1:4, 1:8, 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of Insulin was 107.8%.
Intra-Assay Statistics
Ten replicates of each of four samples containing different Insulin concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Four samples containing different concentrations of Insulin were tested in the independent assays. Acceptable criteria: CV<15%.
Recovery
Insulin was spiked into three human serum samples, and then analyzed. The average recovery of Insulin for serum samples is 103.8%.
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