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Product Overview
Human IL-8 ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-8 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-8Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity3.0 pg/mLAssay Time1.75 hrSample volume50 μLRange6.25 pg/mL-400 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code89/520Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human IL-8 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA004-C01Pre-coated Anti-IL-8 Antibody Microplate1 plateCEA004-C02IL-8 Calibrator20 μg×2CEA004-C03Biotin-Anti-IL-8 Antibody Concentrated Solution100 μLCEA004-C04Biotin Antibody Dilution Buffer8 mLCEA004-C05IL-8 Streptavidin-HRP Concentrated Solution0.5 mLCEA004-C06Streptavidin-HRP Dilution Buffer15 mLCEA004-C0720×Washing Buffer50 mLCEA004-C08Sample Dilution Buffer15 mL×2CEA004-C09Substrate Solution12 mLCEA004-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C004 is less than 3.0 pg/mL.
Sample Values
Serum Sample: Two hundred and forty healthy serum samples were evaluated for the concentrations of human IL-8 in assay.
Dilution Linearity
Three human serum samples with high concentrations of IL-8 were diluted 1:2, 1:4, 1:8, 1:16, 1:32 with Dilution Buffer to produce samples with values within the dynamic range and then assayed. On average, 103.12% of IL-8 was detected from serum samples.
Intra-Assay Statistics
Ten replicates of each of five samples containing different IL-8 concentrations were tested in one assay , Intra-Assay Precision CV＜10%.
Inter-Assay Statistics
Five samples containing different concentrations of IL-8 were tested in three independent assays , Inter-Assay Precision CV<15%.
Recovery
Recombinant IL-8 was spiked into five human serum samples, and then analyzed. On average, 97.54% of IL-8 was recovered from serum samples.
Recombinant IL-8 was spiked into cell supernatant sample, and then analyzed. On average, 98.87% of IL-8 was recovered from serum samples.
Interference effect
Bilirubin (simulated jaundice) concentration should be less than 20mg/dL, hemoglobin (simulated hemolysis) concentration should not be higher than 3500mg/dL, triglyceride (simulated lipemia) concentration may not be higher than 2.0g/L, Heparin concentration should be less than 40U/mL, EDTA concentration should be less than 4mg/mL, and Sodium citrate concentration may not be higher than 40mg/mL, it does not affect the detection result.
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프론티어 진행
의약품 개발 진행- 영어 이름：
Interleukin-8
- 카테고리：
- 상장 의약품 수：
1 상세
- 임상 의약품 수：
6 상세
- 최고 개발 단계：
Approved
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