Human IL-7 ELISA Kit

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제품 번호/규격
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CEA-C216-96tests
$565.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Product Overview

    Human IL-7 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-7 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    IL-7
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    5.0 pg/mL
    Assay Time
    2.75 hr
    Sample volume
    50 μL
    Range
    7.81 pg/mL-500 pg/mL
    Sample Type
    For the quantitative determination of human IL-7 in serum, plasma and cell supernatant.
    NIBSC Code
    90/530, NIBSC/WHO (90/530) approximate value (U/mL) = 0.78 x Clinmax Human IL-7 value (pg/mL)

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant human IL-7 in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow

    IL-7 Workflow

  • Storage

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CEA216-C01
    Pre-coated Anti-IL-7 Antibody Microplate
    1 plate
    CEA216-C02
    Human IL-7 Standard
    5 ng×2
    CEA216-C03
    Biotin-Anti-IL-7 Antibody Con. Solution
    100 μL
    CEA216-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CEA216-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CEA216-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA216-C07
    20×Washing Buffer
    50 mL
    CEA216-C08
    Sample Dilution Buffer
    15 mL×2
    CEA216-C09
    Substrate Solution
    12 mL
    CEA216-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Typical Data 

    Please refer to DS document for the assay protocol.

     IL-7 TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-7 is less than 5 pg/mL.

Validation
  • Intra-Assay Statistics

    Ten replicates of each of three samples containing different IL-7 concentrations were tested in one assay. Acceptable criteria: CV<10%.

     IL-7 INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Three samples containing different concentrations of IL-7 were tested in inde-pendent assays. Acceptable criteria: CV<15%.

     IL-7 INTER-ASSAY STATISTICS
  • Recovery

    Recombinant IL-7 was spiked into three human serum samples, and then analyzed. The average recovery of IL-7 for serum samples is 95.2%.

     IL-7 RECOVERY

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프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Interleukin-7

  • 카테고리:

  • 상장 의약품 수:

    0 상세

  • 임상 의약품 수:

    5 상세

  • 최고 개발 단계:

    Phase 1 Clinical

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