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Product Overview
Mouse IL-2 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant mouse IL-2 that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-2Format96-wells plate breakable into 12 x 8 wells stripsReactivityMouseSensitivity10 pg/mLAssay Time2.75 hrSample volume50 μLRange15.625 pg/mL-1000 pg/mLSample TypeFor the quantitative determination of mouse IL-2, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant mouse IL-2 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow

Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA269-C01Pre-coated Anti-IL-2 Antibody Microplate1 plateCEA269-C02Mouse IL-2 Standard20 ng/mL×4CEA269-C03Biotin-Anti-IL-2 Antibody Con. Solution100 μLCEA269-C04Biotin-Antibody Dilution Buffer15 mLCEA269-C05Streptavidin-HRP Con. Solution500 μLCEA269-C06Streptavidin-HRP Dilution Buffer15 mLCEA269-C0720× Washing Buffer50 mLCEA269-C08Sample Dilution Buffer15 mL×2CEA269-C09Substrate Solution12 mLCEA269-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-2 is less than 10 pg/mL.
Intra-Assay Statistics
Ten replicates of each of 3 samples containing different IL-2 concentrations were tested in one assay. Acceptable criteria: CV<10%.

Inter-Assay Statistics
Three samples containing different concentrations of IL-2 were tested in independent assays. Acceptable criteria: CV<15%.

Recovery
Recombinant IL-2 was spiked into 3 mouse serum samples, and then analyzed. The average recovery of IL-2 for serum samples is 97.0%.

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프론티어 진행
- 영어 이름:
Interleukin-2
- 카테고리:
- 상장 의약품 수:
2 상세
- 임상 의약품 수:
46 상세
- 최고 개발 단계:
Approved
전화
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