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Product Overview
Human IL-17A / CTLA8 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-17A / CTLA8 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-17A/CTLA8Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity<6 pg/mLAssay Time2 hrSample volume50 μLRange31.25 pg/mL-2000 pg/mlSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human IL-17A / CTLA8 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA092-C01Pre-coated Anti-IL-17A Antibody Microplate1 plateCEA092-C02Human IL-17A Standard20 μg×2CEA092-C03Biotin-Anti-IL-17A Antibody Con. Solution300 μLCEA092-C04Biotin-Antibody Dilution Buffer8 mLCEA092-C05Streptavidin-HRP Con. Solution500 μLCEA092-C06Streptavidin-HRP Dilution Buffer15 mLCEA092-C0720×Washing Buffer50 mLCEA092-C08Sample Dilution Buffer15 mL×2CEA092-C09Substrate Solution12 mLCEA092-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-17A is less than 6 pg/mL.
Matrix Effect
Three types of materials were tested to observe if there were matrix effect (interference). If the concentration of hemoglobin (simulated hemolysis) is less than 3500 mg/dL(2%), the concentration of triglyceride (simulated lipid blood) is less than 3 mg/mL, testing results will not be affected.
Dilution Linearity
High concentrations of human IL-17A serum samples were diluted with 1:2, 1:4, 1:8 and 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of IL-17A was 90.14%.
Intra-Assay Statistics
Ten replicates of each of four samples containing different IL-17A concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Five samples containing different concentrations of IL-17A were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant IL-17A was spiked into three human serum samples, and then analyzed. The average recovery of IL-17A for serum samples is 85.37%.
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프론티어 진행
의약품 개발 진행- 영어 이름：
Interleukin 17A
- 카테고리：
- 상장 의약품 수：
5 상세
- 임상 의약품 수：
35 상세
- 최고 개발 단계：
Approved
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