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Product Overview
Human IL-11 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-11 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-11Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity< 3.906 pg/mLRange3.906 pg/mL-250 pg/mLAssay Time3 hr 20 minSample volume50 μLSample TypeFor the quantitative determination of human IL-11 in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human IL-11 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA215-C01Pre-coated Anti-IL-11 Antibody Microplate1 plateCEA215-C02Human IL-11 Standard20 μgCEA215-C03Biotin-Anti-IL-11 Antibody15 μgCEA215-C04Streptavidin-HRP50 μLCEA215-C0510×Washing Buffer50 mLCEA215-C062×Dilution Buffer50 mLCEA215-C07Substrate Solution12 mLCEA215-C08Stop Solution7 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable concentration of IL-11 is less than 3.906 pg/mL.
Sample Values
MRC-5 Medical Research Council cell strain-5 (1.0×10⁶ cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 30ng/mL IL-1β and 30ng/mL TNF-α for 2 days. Subsequent analysis involved quantitative measurement of human IL-11 concentrations in collected culture supernatants using standardized immunoassays.
Intra-Assay Statistics
Ten replicates of each of three samples containing different IL-11 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of IL-11 were tested in inde-pendent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant IL-11 was spiked into three human serum samples, and then ana-lyzed. The average recovery of IL-11 for serum samples is 89.5%.
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프론티어 진행
의약품 개발 진행- 영어 이름：
Interleukin-11
- 카테고리：
- 상장 의약품 수：
1 상세
- 임상 의약품 수：
3 상세
- 최고 개발 단계：
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