Human IgG2 (P331S) Kappa Isotype Control (mAb, carrier free, MALS verified)

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제품 번호/규격
가격
수량
DNP-M885-100ug
$165.00
DNP-M885-1mg (500ug X 2)
$720.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Source

    Human IgG2 (P331S) Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Species

    Human

  • Isotype

    Human IgG2(P331S) | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Specificity

    This product is a specific antibody specifically reacts with DNP.

  • Application

    Application
    Recommended Usage
    ELISA
    0.02-13 ng/mL
  • Purity

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Cross Verification

    This product No cross-reactivity in ELISA with

    Biotinylated Human CD16b (NA2), His,Avitag (Cat. No. CDB-H82Ea).

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    IgG control SDS-PAGE

    Human IgG2 (P331S) Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IgG control SEC-MALS

    The purity of Human IgG2 (P331S) Kappa Isotype Control (mAb) (Cat. No. DNP-M885) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IgG control ELISA

    Immobilized Dinitrophenyl-BSA Conjugate(DNP-BSA) at 10 μg/mL (100 μL/well) can bind Human IgG2 (P331S) Kappa Isotype Control (mAb) (Cat. No. DNP-M885) with a linear range of 0.02-2 ng/mL (QC tested).

    Protocol

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배경 소개

Fc engineered isotype controls are designed to evaluate effector functions such as FcγR binding, FcRn interaction, ADCC, and CDC. Each variant is functionally validated, providing a reliable negative control framework for antibody mechanism studies and therapeutic development.

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