Biotinylated Human IgG1 Kappa Isotype Control (Biotin, mAb, carrier free, MALS verified)

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Cat. No. / Size
Price
Qty
DNP-BM2-25ug
$120.00
DNP-BM2-200ug
$575.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Biotinylated Human IgG1 Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Species

    Human

  • Isotype

    Human IgG1 | Kappa

  • Conjugate

    Biotin

  • Antibody Type

    Recombinant Monoclonal

  • Specificity

    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.

  • Application

    Application
    Recommended Usage
    ELISA
    1-150 ng/mL
  • Purity

    95% as determined by SDS-PAGE.

    95% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IgG control SDS-PAGE

    Biotinylated Human IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    IgG control SEC-MALS

    The purity of Biotinylated Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-BM2) is more than 95% and the molecular weight of this protein is around 145-170 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IgG control ELISA

    Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-BM2) with a linear range of 1-20 ng/mL (QC tested).

    Protocol

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Background

Fluorescently or biotin labeled isotype controls are used to assess non specific staining and autofluorescence in flow cytometry, ELISA, and imaging assays. By recognizing DNP, a hapten absent in biological samples, these controls provide a reliable baseline to distinguish background fluorescence or signal from true antigen specific binding.

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