Product Details
Source
Biotinylated Human IgG1 Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
Species
Human
Isotype
Human IgG1 | Kappa
Conjugate
Biotin
Antibody Type
Recombinant Monoclonal
Specificity
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
Application
ApplicationRecommended UsageELISA1-150 ng/mLPurity
95% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Human IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Biotinylated Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-BM2) is more than 95% and the molecular weight of this protein is around 145-170 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-BM2) with a linear range of 1-20 ng/mL (QC tested).
Protocol
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