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Product Details
Synonym
IBP7, IGFBP7
Source
Human IGFBP-7, Fc Tag (IG7-H5259) is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # Q16270-1).
Predicted N-terminus: Asp 30
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IGFBP-7, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human IGFBP-7, Fc Tag (Cat. No. IG7-H5259) is more than 90% and the molecular weight of this protein is around 102-124 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human C1q R1, His Tag (Cat. No. C11-H5228) at 5 μg/mL (100 μL/well) can bind Human IGFBP-7, Fc Tag (Cat. No. IG7-H5259) with a linear range of 0.6-20 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human IGFBP-7 Protein, Fc Tag (Cat. No. IG7-H5259) immobilized on CM5 Chip can bind Human C1q R1 / CD93 Protein, His Tag (MALS verified) (Cat. No. C11-H5228) with an affinity constant of 7.29 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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