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Product Overview
Mouse IgE ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant Mouse IgE that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalytelgEFormat96-wells plate breakable into 12 x 8 wells stripsReactivityMouseSensitivity70 pg/mLAssay Time2.75 hrSample volume50 μLRange156 pg/mL-10000 pg/mLSample TypeFor the quantitative determination of Mouse IgE in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant mouse Ig E in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow

Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA282-C01Pre-coated Anti-mouse IgE Antibody Microplate1 plateCEA282-C02Mouse IgE Standard5 μg/mL×2CEA282-C03Biotin-Anti-mouse IgE Antibody Con. Solution100 μLCEA282-C04Biotin-Antibody Dilution Buffer8 mLCEA282-C05Streptavidin-HRP Con. Solution500 μLCEA282-C06Streptavidin-HRP Dilution Buffer15 mLCEA282-C0720× Washing Buffer50 mLCEA282-C08Sample Dilution Buffer15 mL×2CEA282-C09Substrate Solution12 mLCEA282-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of mouse IgE is less than 70 pg/mL.
Sample Values
Healthy mouse serum samples (n=24) were evaluated for the concentrations of mouse IgE in the assay. The detectability rate of the samples was 100%, with a mean detectable concentration of 33.02 ng/mL and a concentration range of 3.08 to 83.9 ng/mL.

Intra-Assay Statistics
Ten replicates of each of 3 samples containing different mouse IgE concentrations were tested in one assay. Acceptable criteria: CV<10%.

Inter-Assay Statistics
Three samples containing different concentrations of mouse IgE were tested in independent assays. Acceptable criteria: CV<15%.

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