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Product Overview
Human Erythropoietin (EPO) ELISA Kit (Pre-Aliquoted Standard, Liquid) is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human EPO that is present in biological samples, such as human serum, plasma, and cell culture supernatants. The standard in this kit is provided in liquid form and can be used directly without dilution. Additionally, the standard is supplied in a pre-aliquoted liquid format for immediate use. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteEPOFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity2.0 mIU/mlRange3.125 mIU/ml-200 mIU/mlAssay Time2.75 hrSample volume50 μLSample TypeFor the quantitative determination of human EPO in serum, plasma and cell supernatant.NIBSC Code11/170Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human EPO in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow

Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve.
Intra-Assay Statistics
Ten replicates of each of three samples containing different EPO concentrations were tested in one assay. Acceptable criteria: CV<10%.

Inter-Assay Statistics
Three samples containing different concentrations of EPO were tested in independent assays. Acceptable criteria: CV<15%.

Recovery
Recombinant EPO was spiked into three human serum samples, and then analyzed. The average recovery of EPO for serum samples is 100.1%.

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