제품 세부정보
Synonym
C-X-C motif chemokine 2, Growth-regulated protein beta, Gro-beta, Macrophage inflammatory protein 2-alpha, MIP2-alpha, GRO2, GROB, MIP2A, SCYB2
Source
Human CXCL2, Fc Tag (CX2-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 35 - Asn 107 (Accession # P19875).
Predicted N-terminus: Ala 35
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 34.3 kDa. The protein migrates as 38-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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데이터 표시
SDS-PAGE

Human CXCL2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Human CXCL2, Fc Tag (Cat. No. CX2-H5253) is more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Report
Bioactivity-SPR

Human CXCR2 Protein, Flag,His Tag (Cat. No. CX2-H55D3) immobilized on CM5 Chip can bind Human CXCL2, Fc Tag (Cat. No. CX2-H5253) with an affinity constant of 8.12 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (QC tested).
Protocol
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