Mouse Complement C3a ELISA Kit

사용자 리뷰
제품 번호/규격
가격
수량
CEA-M271-96tests
$565.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Product Overview

    Mouse Complement C3a ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant Mouse Complement C3a that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    2. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    3. Ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    Mouse Complement C3a
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Mouse
    Sensitivity
    30 pg/mL
    Assay Time
    2.75 hr
    Sample volume
    50 μL
    Range
    46 pg/mL-1100 pg/mL
    Sample Type
    For the quantitative determination of Mouse Complement C3a in serum, plasma and cell supernatant.

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant mouse Complement C3a in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow

    Complement C3a Workflow

  • Storage

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CEA271-C01
    Pre-coated Anti-Mouse Complement C3a Antibody Microplate
    1 plate
    CEA271-C02
    Mouse Complement C3a Standard
    2 μg×2
    CEA271-C03
    Biotin-Anti-Mouse Complement C3a Antibody Con. Solution
    50 μL
    CEA271-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CEA271-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CEA271-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA271-C07
    20× Washing Buffer
    50 mL
    CEA271-C08
    Sample Dilution Buffer
    15 mL×2
    CEA271-C09
    Substrate Solution
    12 mL
    CEA271-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

고객님도 확인한 상품

데이터 표시

  • Typical Data 

    Please refer to DS document for the assay protocol.

     Complement C3a TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Mouse Complement C3a is less than 30 pg/mL.

Validation
  • Sample Values

    Serum Sample: Twenty-four healthy mouse serum samples were evaluated for the concentrations of mouse complement C3a in this assay. Mouse complement C3a was detectable in all serum samples (100%), with concentrations ranging from 6 to 19 μg/mL and a mean of 10.15 μg/mL.

     Complement C3a SAMPLE VALUES
  • Intra-Assay Statistics

    Ten replicates of each of three samples containing different Mouse Complement C3a concentrations were tested in one assay. Acceptable criteria: CV<10%.

     Complement C3a INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Three samples containing different concentrations of Mouse Complement C3a were tested in independent assays. Acceptable criteria: CV<15%.

     Complement C3a INTER-ASSAY STATISTICS
  • Recovery

    Recombinant Mouse Complement C3a was spiked into three mouse serum samples, and then analyzed. The average recovery of Mouse Complement C3a for serum samples is 92.5%.

     Complement C3a RECOVERY

사용자 리뷰
댓글 남기기

프론티어 진행

 
연락하기
신약 후보물질 라이선싱
더 많이 알아보기
  • 기본 제품 정보
  • 제품 세부정보
  • 고객님도 확인한 상품
  • 데이터 표시
  • 사용자 리뷰
문의하기

전화
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521

주소 서울특별시 송파구 법원로11길 11, 제3층 제비-314,315호