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Product Overview
Mouse Complement C3a ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant Mouse Complement C3a that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteMouse Complement C3aFormat96-wells plate breakable into 12 x 8 wells stripsReactivityMouseSensitivity30 pg/mLAssay Time2.75 hrSample volume50 μLRange46 pg/mL-1100 pg/mLSample TypeFor the quantitative determination of Mouse Complement C3a in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant mouse Complement C3a in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow

Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA271-C01Pre-coated Anti-Mouse Complement C3a Antibody Microplate1 plateCEA271-C02Mouse Complement C3a Standard2 μg×2CEA271-C03Biotin-Anti-Mouse Complement C3a Antibody Con. Solution50 μLCEA271-C04Biotin-Antibody Dilution Buffer8 mLCEA271-C05Streptavidin-HRP Con. Solution500 μLCEA271-C06Streptavidin-HRP Dilution Buffer15 mLCEA271-C0720× Washing Buffer50 mLCEA271-C08Sample Dilution Buffer15 mL×2CEA271-C09Substrate Solution12 mLCEA271-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Mouse Complement C3a is less than 30 pg/mL.
Sample Values
Serum Sample: Twenty-four healthy mouse serum samples were evaluated for the concentrations of mouse complement C3a in this assay. Mouse complement C3a was detectable in all serum samples (100%), with concentrations ranging from 6 to 19 μg/mL and a mean of 10.15 μg/mL.

Intra-Assay Statistics
Ten replicates of each of three samples containing different Mouse Complement C3a concentrations were tested in one assay. Acceptable criteria: CV<10%.

Inter-Assay Statistics
Three samples containing different concentrations of Mouse Complement C3a were tested in independent assays. Acceptable criteria: CV<15%.

Recovery
Recombinant Mouse Complement C3a was spiked into three mouse serum samples, and then analyzed. The average recovery of Mouse Complement C3a for serum samples is 92.5%.

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