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Product Details
Synonym
CD38, T10, cADPr hydrolase 1
Source
Human CD38, Mouse IgG2a Fc Tag (CD8-H5253) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # P28907-1).
Predicted N-terminus: Val 43
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 56.9 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD38, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human CD38, Mouse IgG2a Fc Tag (Cat. No. CD8-H5253) at 5 μg/mL (100 μL/well) can bind Anti-CD38 MAb, Human IgG1 with a linear range of 0.4-2 ng/mL (QC tested).
Protocol
Bioactivity-FACS
2e5 of Anti-CD38 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human CD38, Mouse IgG2a Fc Tag (Cat. No. CD8-H5253) and negative control protein respectively, washed and then followed by PE anti-mouse IgG2a Antibody and analyzed with FACS (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Lymphocyte differentiation antigen CD38
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
47 Details
- Highest Development Stage：
Approved
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