Human CD30 / TNFRSF8 Protein, His Tag, low endotoxin

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Cat. No. / Size
Price
Qty
CD0-H52H5-100ug
$270.00
CD0-H52H5-1mg
$1700.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    TNFRSF8, CD30, D1S166E, Ki-1

  • Source

    Human CD30, His Tag (CD0-H52H5) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).

    Predicted N-terminus: Phe 19

    Request for sequence
  • Molecular Characterization

    CD30 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 40.4 kDa. The protein migrates as 53-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD30 SDS-PAGE

    Human CD30, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     CD30 ELISA

    Immobilized Human CD30, His Tag (Cat. No. CD0-H52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD30 Human Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).

    Protocol
  •  CD30 ELISA

    Immobilized Monoclonal Anti-Human CD30 Human Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Human CD30, His Tag (Cat. No. CD0-H52H5) with a linear range of 0.6-5 ng/mL (Routinely tested).

    Protocol

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Background

Human CD30 is also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. TNFRSF-8 is expressed by activated, but not by resting, T and B cells. Also, CD30 is expressed on classical Hodgkin Lymphoma cells together with CD15. CD30 is the receptor for TNFSF8/CD30L. CD30 can interact with TRAF2 and TRAF5, and mediate the signal transduction that leads to the activation of NF-kappa-B. TNFRSF8 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.

Recent Advances

 
Drug Development Progress
  • English Name:

    Tumor necrosis factor receptor superfamily member 8

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    22 Details

  • Highest Development Stage:

    Approved

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