제품 세부정보
Materials Provided
IDComponentsSizeBIS003-C01Pre-coated Human CD20 Protein Microplate1 plate (8×12 strips)BIS003-C02Anti-CD3 & CD20 Antibody Standard20 μgBIS003-C03HRP-Human CD3E Protein20 μgBIS003-C0410×Washing Buffer50 mLBIS003-C052×Dilution Buffer50 mLBIS003-C06Substrate Solution12 mLBIS003-C07Stop Solution7 mLProduct Overview
The Bispecific CD20:CD3 Bridging ELISA Kit is a comprehensive, ready-to-use assay designed to functionally confirm the simultaneous binding of anti-CD20-anti-CD3 bispecific antibodies (BsAbs) to both of their intended targets. This assay quantifies the formation of the critical CD20-BsAb-CD3 trimolecular complex, providing a direct measure of the antibody's primary mechanism of action. This all-inclusive kit contains all essential reagents for 100 reactions, including recombinant human CD20, a CD3-containing detection reagent, and a validated positive control antibody, thereby streamlining the screening and profiling of therapeutic candidates with exceptional precision and reliability.
Storage
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.
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Typical Data
Please refer to DS document for the assay protocol.

Detection of anti-CD3 & CD20 antibodies by Bridging ELISA Assay.
Bispecific Human CD20 & CD3E Bridging ELISA Kit can specifically identify Anti-CD3 & CD20 Antibodies, with linear range of 0.020-1.289 nM (QC tested).
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