제품 세부정보
Materials Provided
IDComponentsSizeBIS002-C01Pre-coated Human CD3E & CD3G Protein Microplate1 plate (8×12 strips)BIS002-C02Anti-CD3 & CD19 Antibody Standard20 μgBIS002-C03Biotin-Human CD19 Protein20 μgBIS002-C04Streptavidin-HRP20 μgBIS002-C0510×Washing Buffer50 mLBIS002-C062×Dilution Buffer50 mLBIS002-C07Substrate Solution12 mLBIS002-C08Stop Solution7 mLProduct Overview
The Bispecific CD19:CD3 Bridging ELISA Kit is a comprehensive, ready-to-use assay designed to functionally confirm the simultaneous binding of anti-CD19-anti-CD3 bispecific antibodies (BsAbs) to both of their intended targets.This assay quantifies the formation of the critical CD19-BsAb-CD3 trimolecular complex, providing a direct measure of the antibody's primary mechanism of action. This all-inclusive kit contains all essential reagents for 100 reactions, including recombinant human CD19, a CD3-containing detection reagent, and a validated positive control antibody, thereby streamlining the screening and profiling of therapeutic candidates with exceptional precision and reliability.
Storage
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.
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Typical Data
Please refer to DS document for the assay protocol.

Detection of anti-CD3 & CD19 antibodies by Bridging ELISA Assay.
Bispecific Human CD19 & CD3 Bridging ELISA Kit can specifically identify Anti-CD3 & CD19 Antibodies, with linear range of 0.090-5.787 nM (QC tested).
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